Fig. 6

C2orf40 could down-regulate the expression levels of homologous recombination-associated proteins and inhibit the activation of PI3K/Akt/mTOR signaling pathway. A‒D Correlation analysis revealed that the C2orf40 expression level was negatively correlated with the expression levels of BRCA1 (A), BRCA2 (B), CDC25A (C), and RAD51 (D). The data were obtained from three GEO datasets (GSE12452, GSE53819, and GSE64634). E‒F The representative images (E) and statistical analysis (F) of Western blotting of the expression levels of homologous recombination-associated proteins (BRCA1, BRCA2, RAD51, and CDC25A) in HONE-1 and SUNE-1 cells transiently transfected with C2orf40-overexpressed plasmids. β-actin was used as an internal reference. G‒H The representative images. (G) and statistical analysis (H) of the activation of the PI3K/Akt/mTOR signaling pathway. HONE-1 and SUNE-1 cells were transiently transfected with C2orf40 overexpression vectors or empty vectors, and exposed to 0 or 2 Gy radiation. The experiments were performed in triplicate and repeated twice. Data are presented as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001