Fig. 5
From: St13 protects against disordered acinar cell arachidonic acid pathway in chronic pancreatitis
Sdf2l1 inhibition exacerbated lipid accumulation, inflammation and ER stress in CP. shSdf2l1 was delivered to the pancreas of PRSS1Tg mice to silence Sdf2l1. A Histological alterations and collagen deposition were assessed by H&E and Masson’s trichrome, respectively, in pancreatic tissues from caerulein-treated and ethanol-treated PRSS1Tg mice. B LC–MS was performed to measure the abundance of 3 AA metabolites (PGE2, LTB4, 8(9)-EET) in pancreatic tissues of PRSS1Tg mice. C Ultrastructural changes in pancreatic tissues from PRSS1Tg mice treated or untreated with Cer/EtOH were analysed by TEM; black arrows (↑): cell nuclei; white arrows (↑): ERs; blue arrows (↑): zymogen granules; purple arrows (↑): mitochondria; orange arrows (↑): fibrin; red arrows (↑): lipid droplets. D qRT-PCR and (E) Western blot analyses of IRE1α, XBP1s, COX-2, Sdf2l1, and PGE2 expression in pancreatic tissues from PRSS1Tg mice;GaPDH was used as an internal loading control. Scale bars (H&E and Masson), 100 μm. NC, negative control. ns no significant difference; *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001. The data are presented as the means ± SDs