Fig. 3From: The role of neutrophil elastase in aortic valve calcificationThe effect of NE on pAVIC calcification induced by osteogenic medium. pVICs were cultured in osteogenic medium combined with NE, Alvelestat or both for 24 h. A, B RT-qPCR detection of RUNX2 and OPN. C–H Western blot analysis of bcl-2, bax, TNF-α, RUNX2 and OPN. I The degree of osteoblastic differentiation was measured by ALP staining. J Calcium deposit was measured by Alizarin Red Staining. Scar bar = 100 µm. *P < 0.05, **P < 0.01, ****P < 0.0001 vs corresponding control groups. NS meant no significant difference. The measurement data are expressed as the mean ± SD. All experiments were independently repeated three timesBack to article page