Fig. 6

Examination of assay precision across instruments of cryopreserved CAR/TCR T-cell flow cytometry quality controls. The cryopreserved flow cytometry quality control cells were thawed, stained and measured on two flow instruments: BD FACSCanto II (Canto II) and BD FACSCanto 10-Color (Canto X) at all time points across the one-year long-term stability study as described above. The both instruments were used to assess cell viability as well as the expression of the following CAR and TCR engineered T-cell surface markers: protein L, CD22-Fc (Siglec-2) and CD19-Fc for CD19/CD22 bispecific CAR T-cell (A), EGFR for FGFR4 CAR T-cell (B) and murine TCR-beta for KK-LC-1 TCR T-cell (C). Data are shown as mean ± SD. The mean values of transduction efficiency and identity markers of each quality control cells between Canto II and Canto X are summarized in each table