Fig. 1

Generation of CAR/TCR T-cell flow cytometry quality control cells. Cells from two CAR T-cell products: CD19/CD22 bispecific CAR T-cell (A) and FGFR4 CAR T-cell (B) and one TCR engineered T-cell product: KK-LC-1 TCR T-cell (C) were harvested and stained. Flow cytometry was used to measure cell viability; CD3, CD4 and CD8 frequencies; and the expression of CAR/TCR T-cell markers: protein L, CD22-Fc (Siglec-2) and CD19-Fc for CD19/CD22 bispecific CAR T-cell, EGFR for FGFR4 CAR T-cell and murine TCR-beta for KK-LC-1 TCR T-cell. Data are shown as mean ± SD. The representative flow cytometry histograms were gated on viable CD3⁺ cells showing the results of analysis for protein L, CD22-Fc (Siglec-2), CD19-Fc (A), for EGFR (B) and for murine TCR-beta (C). Results from analysis of untransduced (UT) cells are shown in red and transduced (TR) cells are shown in blue in flow cytometry histogram. The mean values of each parameter for three quality control cells are summarized in each table