Fig. 4
From: TRPC3, but not TRPC1, as a good therapeutic target for standalone or complementary treatment of DMD
Subcellular localization of TRPC1 and TRPC3 protein expression in EDL muscle fibers from WT and DMDmdx rats. A and B Confocal images acquired in longitudinal axis and at the largest diameter of EDL muscle fibers from WT and DMDmdx rats of 4.5 months of age. Fibers were fixed, permeabilized and co-labeled by immunofluorescence to highlight TRPC1 or TRPC3 protein expression and compare it to RyR and Cav-3 ones. A and B are montages of pictures cropped from original images acquired using a X60 objective. Scale bar are reported in white and corresponds to 14 µm. C and D TRPC1 and TRPC3, respectively, sub-peripheral sarcolemma area (peripheral sarcolemma labeled using Cav-3 antibodies and 5 µm beneath area) to fiber center (the rest of the intracellular area) density signal ratio. Each bar represents mean ± SEM value of density ratios measured in n muscle EDL muscle fibers and N animals (n/N are noted at the bottom of each bar). Potential significant differences were evaluated using Student’s unpaired t-test