Fig. 5

Matrix stiffness mediated by CAFs-derived LOX promotes OSCC progression via FAK signaling pathway. A The protein expression of p-FAK and FAK were measured by western blot assay in Cal27 cells cultured on collagen gels. B, C, The proliferation ability of Cal27 cells on collagen gels after FAKi treatment was measured by CCk-8 assay. D The invasion ability of Cal27 cells after FAKi treatment was measured via transwell chambers coated with collagen gels. Representative images and quantification data were shown. E The protein expression of p-FAK, FAK and EMT-related markers were measured by western blot assay in Cal27 cells after FAKi treatment. β-actin served as loading control. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001