Fig. 3

Proteomic comparison of sex-specific changes after ischemia. A Results of differential expression analysis. Proteins under sex-specific regulation are highlighted. An adjusted P-value of < 0.05 was considered significant compared to the sex-matched control group. The female proteomic adaptation following ischemia is characterized by a higher number of upregulated proteins compared to males. B sPLS-DA analysis on the proteomes of all experimental groups. The supervised method clearly separated all groups, proving that both sex and ischemia affect proteomic profiles. C Proteins under sex-dependent regulation in response to ischemia were grouped according to biological categories. Statistically significant association was found between sex and differential expression of proteins with a function in transcription, inflammation, extracellular remodeling and cytoskeletal organization. Statistical significance of Fisher’s exact test is highlighted as follows: *P < 0.05, **P < 0.01, ***P < 0.001. M-Co: male control; M-Isch: male ischemic; F-Co: female control; F-Isch: female ischemic