Fig. 1
From: Diagnostic performance of Oncuria™, a urinalysis test for bladder cancer
Flow diagram of phases project. Gene expression profiling (Affymetrix U133 Plus 2.0 arrays) followed by quantitative PCR verification, and glycoprotein profiling (dual-lectin affinity chromatography and liquid chromatography/tandem mass spectrometry) followed by Western blot analysis or ELISA verification were used to discover and validate RNA and protein expression profiles associated with bladder cancer. Data integration informed the selection of a 19-biomarker panel for testing which was narrowed to 10 protein biomarkers which has been validated in independent cohorts using commercial ELISA assays or custom-designed multiplex assay. The resulting Oncuria™assay used in this study has been analytically validated