Fig. 4

Effect of circPLXNB2 expression on the levels of PLXNB2 and cell cycle-related proteins. a CircPLXNB2 OE significantly decreased the proportion of HL-60 cells in G0/G1 phase and increased the proportion of HL-60 cells in S phase. b The circPLXNB2 shRNA significantly increased the proportion of OCI-AML3 cells in G0/G1 phase and decreased the proportion of OCI-AML3 cells in S phase. c Representative FCM plots of the effects of circPLXNB2 expression on cell cycle progression. The expression of circPLXNB2 (d) and the PLXNB2 mRNA (e) was detected using qRT-PCR. f Representative Western blots showing the expression of BCL2, BAX, cyclin D1 and PLXNB2 in AML cells; β-actin was used as a loading control. Nontransfected cells were used as a control, and the vector or shRNA NC was used as a negative control. Each experiment was repeated three times. ***P < 0.001 compared with the control. CircPLXNB2 OE circPLXNB2 overexpression, circPLXNB2 shRNA circPLXNB2 short hairpin RNA, NC negative control