Table 4 qPCR-validation of selected genes from RNASeq
Gene symbol | NASH-STZ vs NASH | NASH-STZ-HI vs NASH-STZ | ||||||
|---|---|---|---|---|---|---|---|---|
| Â | RNASeq (log2-fold change) | q-value | qPCR (log2-fold change) | q-value | RNASeq (log2-fold change) | q-value | qPCR (log2-fold change) | q-value |
Gpnmb | 5.43 | 2.00 × E−03 | 5.55 | 2.94 × E−05 | − 2.92 | 2.96 × E−03 | − 2.65 | 1.43 × E−02 |
Pklr | − 3.35 | 3.10 × E−03 | − 3.58 | 3.22 × E−04 | 3.25 | 3.65 × E−03 | 3.55 | 3.48 × E−04 |
Spp1 | 4.31 | 2.14 × E−03 | 4.74 | 1.01 × E−05 | − 3.01 | 3.77 × E−03 | − 3.04 | 9.20 × E−04 |
Soat1 | 3.64 | 1.02 × E−03 | 3.62 | 1.71 × E−06 | − 2.84 | 2.28 × E−03 | − 2.50 | 1.14 × E−04 |
Ccl2 | 2.78 | 4.76 × E−03 | 3.19 | 1.25 × E−03 | − 1.96 | 7.58 × E−03 | − 1.72 | 5.10 × E−02 |
Il1β | 1.72 | < 0.05 | 1.54 | 4.16 × E−03 | − 1.21 | < 0.05 | − 0.82 | 9.31 × E−02 |
Col3a1 | 1.64 | 1.93 × E−03 | 1.70 | 7.17 × E−05 | − 0.96 | 5.06 × E−03 | − 0.94 | 9.69 × E−03 |
Timp1 | 2.71 | 3.20 × E−03 | 2.69 | 3.13 × E−04 | − 1.69 | 2.18 × E−03 | − 1.57 | 1.70 × E−02 |