Fig. 4

Long-term L. edodes derived β-glucan supplementation inhibited the microgliosis and neuroinflammation in the hippocampus of mice fed HF diet. a The representative image of immunohistochemistry staining of Iba1 in the cornus ammonis (CA1), CA3 and dentate gyrus (DG) regions of hippocampus, b the statistical results of Iba1⁺ positive cells in mentioned regions. c mRNA expression of TNF-α, IL-6 and IL-1β in the hippocampus. Data are presented as mean ± SEM. n = 3 per group. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, vs lab chow diet group (LC); ^#P < 0.05, ^##P < 0.01, ^###P < 0.001, vs high-fat diet group (HF). HFL, L. edodes β-glucan supplementation in HF diet group