Fig. 2

Secretion profile and in vitro DC culture. a Cell culture supernatants were collected and analyzed using the Procartaplex™ multiplex immunoassay. Finally, cytokine and chemokine concentration were determined from three independent experiments. Given are the mean + SD. *p < 0.05; **p < 0.01; unpaired one-sided T-test. b Left panel: representative flow cytometry of DCs and right panel: quantitative phenotyping of DCs taken from three individual mice. Non-adherent cells were analyzed. Given are the % numbers of positive cells measured upon gating on viable cells (n  =  3 mice). c Representative microscopic images of DCs loaded with tumor A7450 T1 M1 or 328 tumor lysate, respectively. Original magnification 10×. d Phenotyping of peripheral blood mononuclear cells after co-culture with loaded DCs (DC:immune cell ratio: 1:10). On the 5th day, Brefeldin A was used to enhance intracellular cytokine staining signals. Immunophenotypic changes were determined using flow cytometry, data analyses were performed using BD FACSuite software. Given are the % numbers of positive cells measured upon gating on viable cells. *p < 0.05; **p < 0.01; ***p < 0.001; one-way ANOVA (Bonferroni multiple comparison)