Fig. 6
From: Novel single-domain antibodies against the EGFR domain III epitope exhibit the anti-tumor effect
Inhibition of A549 xenograft growth in BALB/c nude mice by anti-EGFR sdAbs. A total of 5 × 106 A549 cells were injected into the right flank of each mouse. SdAbs were injected after tumor sizes reached to about 100 mm3. a Mice were injected with 10 mg/kg anti-EGFR sdAbs (aEG2E12 and aEG4D9) every 3 days. PBS, DDP (2 mg/kg) and two sdAbs (aVE201 and aHer2-13C1, 10 mg/kg) were included as controls. b, c Mice were sacrificed on the 24th day following the first injections of the test reagents, and tumors were removed, photographed and weighted. d Tumor sections were stained by HE or examined with anti-Ki67, anti-CD31 and anti-caspase 3 antibodies. e Integral optical density (IOD) was calculated from d by Image-Pro Plus analysis software. Data are shown as mean ± S.D. (n = 4). *P < 0.05 and **P < 0.01 vs. the respective PBS control