Expression and clinical significance of LAG-3, FGL1, PD-L1 and CD8+T cells in hepatocellular carcinoma using multiplex quantitative analysis

Table 2 Univariate and multivariate analysis for OS

Variables	OS			PFS
	Univariate	Multivariate	p value	Univariate	Multivariate	p value
	p value	HR(95% CI)	p value	p value	HR(95% CI)	p value
Age, years(> 50 vs. ≤ 50)	0.911		NA	0.713		NA
Gender(male vs. female)	0.873		NA	0.115		NA
HBsAg (positive vs. negative)	0.065		NS	0.001	3.717(1.504–9.185)	0.004
AFP, ng/ml (> 20 vs. ≤ 20)	0.094		NS	0.262		NA
Cirrhosis (yes vs. no)	0.036	2.098(1.240–3.550)	0.006	0.018	2.217(1.441–3.412)	0.000
Tumor number (multiple vs. single)	0.122		NA	0.001	1.530(1.182–1.981)	0.001
Tumor size, cm (> 5 vs. ≤ 5)	0.005	1.893(1.059–3.384)	0.031	0.000	2.690(1.693–4.275)	0.000
Tumor differentiation (III vs. I-II)	0.124		NA	0.111		NA
vascular invasion (present vs. absent)	0.000	4.171(1.830–9.504)	0.001	0.038		NS
LAG-3 expression (high vs. low)	0.009	1.879(1.080–3.267)	0.025	0.012	1.642(1.058–2.549)	0.027
CD8 expression (high vs. low)	0.007	0.562(0.330–0.957)	0.034	0.000	0.618(0.400–0.956)	0.031
PD-L1 status on TC (positive vs. negative)	0.122		NA	0.065		NS
PD-L1 status on IC (positive vs. negative)	0.938		NA	0.074		NS
FGL1 status on TC (positive vs. negative)	0.489		NA	0.968		NA
FGL1 status on IC (positive vs. negative)	0.144		NA	0.584		NA

P < 0.05 was considered as statistically significant
HOS overall survival, DFS disease-free survival, HBsAg hepatitis B surface antigen, AFP αfetoprotein, HR hazard ratio, CI confidential interval, NA not adopted, NS not significant, TC tumor cells, IC immune cells

ISSN: 1479-5876