Fig. 3

Cyt-1, Cyt-2, and IL-10 mRNA quantification after in vitro anti-CD3/CD46 MoAb stimulation. mRNA levels of Cyt-1 (a), Cyt-2 (b), Cyt-2 and Cyt-1 ratio (c), and IL-10 mRNA levels (d) in CD4⁺ cells pre- and post- in vitro stimulation. CD4⁺ cells obtained from IFN-β-treated patients before therapy initiation (T0) and then after 12 (T12) and 24 (T24) months of therapy and healthy controls (Ctrl) and stimulated with anti-CD3 and anti-CD46 MoAbs. Results are calculated as normalization ratio (NR), relative to a common standard sample. Estimated means are shown, connected by continuous lines, along with error bars representing the 95% confidence interval. Other lines indicate statistical significance