Fig. 3
From: High-intensity interval training in allogeneic adoptive T-cell immunotherapy – a big HIT?
Changes in frequencies of VSTs between moderately and highly fit donors. Donors were additionally classified into a moderately and a highly fit group according to the International Physical Activity Questionnaire (IPAQ). Frequencies of functional-active virus-specific T cells were determined by IFN-γ EliSpot assay. Negative and positive controls were carried out by using either medium or 1 μg/ml staphylococcal enterotoxins B (SEB). Results a in response to CMV, b EBV and c AdV from before and 24 h after exercise are shown as fold changes of spots per well per 1000 CD3+ T cells after subtracting those of the respective negative control. Positive control showed a positive result for all analysed samples detected as TNTC (too numerous to count, TNTC ≥ 1000 spw). Results are displayed as mean ± SD