Fig. 5

Role of mitophagy in the protective effect of PD against sepsis-induced MD. The mice were subjected to CLP and administered vehicle, PD (30 mg/kg), or PD plus mdivi-1 (3 mg/kg). Parkin−/− mice were subjected to CLP and administered PD (30 mg/kg). The mice were sacrificed at 12 h after CLP. a RTECs were isolated and stained with JC-1 to measure mitochondrial membrane potential (MMP) using a laser confocal-scanning microscope. Scale bar: 50 μm. b Quantification of the intracellular red and green fluorescence emitted by JC-1. c The cellular ATP levels of RTECs were measured by a luciferase-based assay