Fig. 1
Gating Strategy and cell subset frequencies of terminal ileum tissue-resident memory CD4+ T (TRM) cells. Terminal ileum LPMC CD4+ tissue resident T memory cell (TRM) subsets in representative volunteers were detected in a Unvaccinated and Ty21a-vaccinated participants by expression of CD69 and CD103 markers following the gating strategy shown in the figure. b Frequencies of CD4+ CD103+ TRM and CD4+ CD103− TRM subsets were measured and compared between TI LPMC obtained from Ty21a-vaccinated (n = 18; red symbols) and unvaccinated volunteers (n = 18; black symbols) with significant differences (*p < 0.05) and a trend is indicated by its p-value. Horizontal bars represent median values