Fig. 2

TCs were identified with morphology observation and CD34/PDGFRα/vimentin positive in culture. a Representative photos of TCs cultured for 0 h to 48 h captured by Cell-IQ, respectively. Original magnification ×100. Immunofluorescence labelling for vimentin (b, green), PDGFRα (c, red) and CD34 (d, purple) with DAPI (e, blue) counterstain for nuclei. TCs are CD34, PDGFRα and vimentin positive (f). Arrows and arrow heads show typical TCs with long and thin telopodes (Tp) with dilations. Original magnification ×400. The ultrastructure of mouse TCs are shown in g and h. Tp: telopode; nu: nucleolus; Mt: mitochondria