Fig. 3

FASLG contained a potential miR-21 binding sites. a Sequence alignment of miR-21 with the putative binding sites in the FASLG gene, as detected by TargetScan. FASLG mutant indicated the FASLG 3′UTR with mutations in miR-21 binding region. b Luciferase report assays of constructs with FASLG 3′UTR or mutated FASLG 3′UTR in the absence or presence of the indicated miRNAs. c Western blot detection of FASLG protein in EPCs transfected with different duplexes. (Left panel) quantitative analysis of FASLG protein expression. (Right panel) d the protein level of FASLG in EPCs transfected with NC agomir, miR-21 agomir and FASLG, or FASLG siRNA, respectively. (Left panel) quantitative analysis of FASLG protein expression. (Right panel) β-actin served as internal control. *p < 0.05 vs. negative control