Fig. 2

The effect of miR-21 on proliferation and angiogenesis on EPCs. a CCK-8 assay of proliferation of EPCs at 24, 48 and 96 h after transfection with NC agomir, miR-21 agomir, NC antagomir or miR-21 antagomir, respectively and miR-21 overexpression could increase EPCs proliferation. b In vitro tube formation assay was used to determine the angiogenesis ability of EPCs within different groups. MiR-21 overexpression and inhibition could increase and decrease angiogenesis in vitro, respectively. Relative tube number and length (×100) (n = 3). c Quantification of tube formation and length in different groups. *p < 0.05 vs. negative control