Fig. 3

Cells characterization by FC. a BMCs characterization. HSCs (CD34+ cells) analysis was performed by FC according to the ISHAGE reference method. b Immunophenotype characterization of isolated MSCs and S-MSCs. MSCs and S-MSCs (grey) were stained with the following human monoclonal antibodies: CD105 (endoglin), CD90 (Thy-1), CD73 (5′ nucleotidase), CD34, CD45, CD11b (integrin alpha M), HLA-DR (human leucocyte antigen-D related), CD146 (MCAM), CD140a (PDGF RA), CD140b (PDGF RB), CD31 (PECAM1), CD144 (Ve-Cadherin), VEGF R1, VEGF-R2, CD184 (CXCR4) and CD106 (VCAM1). The isotype-matched mouse IgG1-FITC, IgG1-PE, IgG1-PCy5 and IgG1- PCy7 were used as negative controls (empty)