Fig. 2

Particles size and number distribution. Correlation between the number of particles isolated and the serum volume (a). The NTA size distribution shows that PEG enriched a higher number of total EVs in 0.5, 1 and 2 ml (b). Total number of particles per ml quantification (c). EV size distribution (nm) (d). Quantitative ExoELISA assay of tetraspanin CD9. Data are shown as particles per ml (e). *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001. Data shown as mean ± S.D. Experiments were performed in duplicate and repeated three times