Fig. 1

Illustration of the CAR architectures and functionality of CAR in vitro. A Cartoon illustration of each CAR used in the study: PSMA-CAR (a), IL23mAb-T2A-PSMA-CAR (b), IL23mAb-PSMA-CAR (c), and PSMA-CAR (soluble IL23mAb) (d). B Gene structure of CARs used in study: PSMA-CAR (a), IL23mAb-T2A-PSMA-CAR (b), IL23mAb-PSMA-CAR (c), and PSMA-CAR (soluble IL23mAb) (d). CD8 ectodomain (CD8EC), CD8 transmembrane domain (TM), 4-1BB costimulatory domain, and CD3ζ T cell signaling chain (CD3zeta). C Lentiviral transduction allows for efficient expression of different CARs in primary human T cells. In the upper lane, un-transduced cells (CAR negative cells) were labelled in red. Secondary antibody control was labelled in blue. Isotype control was in yellow and transduced cells were in green. In the under lane, un-transduced cells were labelled in red. Secondary antibody control was labelled in blue, and transduced cells were in yellow. D Specificity of lysis by different CAR T cells in PSMA + PC3 cells. E Antigen-specific proliferation of CAR T cells upon co-culture with PSMA + PC3 cells. F Cytokine analysis of different T cell supernatants. The error bars represent ± SD