Fig. 1

Positions of single amino acid substitution of the RhD protein (adapted from Flegel [4] and Srivastava [45]). There are 417 amino acids in the RhD protein, shown here as circles. The mature protein in the cell membrane lacks the first amino acid. The nine exon boundaries in the RHD cDNA as reflected in the amino acid sequence are labeled as black bars. All detected amino acid substitutions encoding D variant alleles are labeled as colored circles. A synonymous single nucleotide polymorphism (SNP) caused no amino acid change (gray). The other SNPs are nonsynonymous and cause amino acid changes that are predicted to affect the RhD protein structure (red) or to be neutral (blue)