Fig. 4

Impact of albumin supplementation when used as cryoprotectant, or additive, or both. Cryopreservation of MSC was performed using either AlbIX or HSA and followed by thawing and conditioning with either one of the two albumins generating the 4 possible combinations (HSA:HSA, HSA:AlbIX, AlbIX:AlbIX, and AlbIX:HSA). Viability pre- and post-cryopreservation shows higher viability rates in samples supplemented with AlbIX (a). Viability of all four conditions were followed up for 72 h (b). Data resulting from these experiments have been grouped according to the cryoprotectant used, either HSA (c) or AlbIX (d). In e, viability reduction is presented for all conditions along time, namely: control (HSA:HSA), as a cryoprotectant (AlbIX:HSA), as a stabilizer post-thawing (HSA:AlbIX), or both (AlbIX:AlbIX)