Fig. 3

Retention of viability, phenotypic profile and multipotentiality of MSC after cryopreservation in the presence of either HSA or recombinant albumin. a Post-thawing stability assessed by flow cytometry showing the mean of the percentage of viability (%) along the stability follow up time using HSA (in black) or AlbIX (in grey) additives (n = 6 cell lines), percentage of reduction and results of ANOVA Tukey Multiple comparison test are showed; Phenotypic characterisation of MSC at times 0 and 72 h. Finally, in b, in vitro differentiation assays were performed to confirm osteogenic, adipogenic and chondrogenic potential of MSC and the outcome of each differentiation were assessed by specific stainings: alkaline phosphatase (ALP) and Alizarin Red (AR) for bone tissue, Oil Red O for fat; and Safranin O, for cartilage