Fig. 4

STAT3 mediates EMT reversal induced by YM155 independently of survivin. a Morphology of U251 and U87 cells before and after knockdown with survivin siRNA, or knockdown with YM155 treatment. b Phospho-kinase array of 43 phosphorylated kinases in U251 cells treated with 5 nM YM155 relative to DMSO control. c Graphic representation of the quantitation of 10 molecules with the most significant difference in phosphorylation status between YM155 treatment and control. d Western blot analysis of pSTAT3 (Ser727), pSTAT3 (Tyr705), STAT3, Cyclin D1 and c-Myc of U251 and U87 cells incubated with increasing concentrations of YM155. e Western blot analysis of pSTAT3 (Ser727), pSTAT3 (Tyr705), STAT3, N-cadherin and E-cadherin in U251 and U87 cells treated with DMSO, radiation, and combination treatment (YM155 5 nM + radiation 4 Gy)