Fig. 5

Phenotype detections. a The intracellular localization of P53 was analyzed using confocal fluorescence microscopy. The fibroblast cells from P53 KO piglets and WT piglets were treated with DOX at 100 µM for 24 h and stained with Hoechst 33342 (blue) and an anti-P53 antibody (green). b The fibroblast cells were treated as described above and protein expression levels were examined by Western blotting. P53 and P21 protein expression in the treated fibroblasts are shown in cropped blots using an anti-P53 and anti-P21 monoclonal antibody. Anti-GAPDH served as a loading control