Identification of new MUC1 epitopes using HLA-transgenic animals: implication for immunomonitoring

Table 2 Compilation of peptide identification and validations methods

Mouse HLA	Peptide	Tetramer		In vivo kill
Mouse HLA	Peptide	Fully human	Chimeric	In vivo kill
HLA-A*01	is E mflqi Y	Neg	ND	Neg
HLA-A*02	stappvhn V	Neg	ND	Neg
	s L sytnpa V	4/6	4/4	Neg
	l L ltvlt V	1/6	4/4	Neg
	v L vcvlva L	Neg	ND	4/5
HLA-B*07	a P dnrpa L	11/11	5/5	5/5
HLA-B*27	r R knygqldi F	Neg	ND	Neg
HLA-B*35	f P ardtyhp M	Neg	ND	8/8
HLA-C*7	difp A rdt Y	Neg	ND	Neg

All pMHC gave above background IFNg-Elispot in an in vitro recall response in at least three independent experiments. For tetramer staining and in vivo kill assay, the number of positive results (number of mice positive/total number of mice analyzed). Anchor residues are in bold
Neg negative results, ND not done

ISSN: 1479-5876