Fig. 3

The effect of IPreC, IPostC and RIC on myocardial necrosis (a; n = 5–6/group) and AAR (b; n = 5–6/group) as evaluated with conventional TTC and Evans blue staining. c Representative images of Evans blue/TTC-stained heart sections from three different hearts indicating myocardial necrosis and AAR (Each image was taken from the apical side of the third 1-cm slice. Images are optimized for visualization of TTC staining in the InfarctSize software.). Orange slice outline, green ventricular chamber, purple AAR, yellow myocardial necrosis. *p < 0.05 vs. Isch. Isch ischemia only, IPreC ischemic preconditioning, IPostC ischemic postconditioning, RIC remote ischemic conditioning, AAR area at risk, TTC triphenyl tetrazolium chloride, MRI LV left ventricle