Fig. 1

Representative immunohistochemistry images of hMLH1, hMSH-2 and EGFR protein expression. Nuclear hMLH1 staining in tumours with “low” (a) and “normal” (b) expression. Representative hMSH-2 staining in tumour with “low” (c) and “normal” (d) expression. A seminoma (e) and embryonal carcinoma (f) showing positivity for EGFR staining. A high-power view of the boxed regions is shown in the insets. Nuclear and membrane signal (brown) is visualized with diamino-benzidine as chromogen. The black arrowhead depict EGFR immunoreactivity in the membrane of a seminoma cell. The red arrowhead shows nuclear hMLH1 signal