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Table 2 Comparison of the composition of PBMC concentrates and the elutriated lymphocyte fraction used to manufacture CD19- and GD2-CAR T cells

From: Elutriated lymphocytes for manufacturing chimeric antigen receptor T cells

Cell type

CAR T cell type

CD19

GD2

All

n

PBMC

Elutriated lymphs

n

PBMC

Elutriated Lymphs

n

PBMC

Elutriated lymphs

CD3+

8

49.0 ± 23.1

62.1 ± 24.4a

5

42.2 ± 17.0

58.0 ± 23.0a

13

49.3 ± 22.5

64.6 ± 25.5a

CD14+

8

17.2 ± 11.4

3.5 ± 1.7a

4

20.9 ± 9.7

3.9 ± 3.2a

12

19.9 ± 13.7

3.6 ± 4.8a

CD15+

8

8.2 ± 15.2

4.1 ± 10.6

4

3.9 ± 2.8

0.8 ± 1.4

12

6.9 ± 12.0

2.9 ± 8.2a

CD19+

8

13.8 ± 16.9

13.4 ± 16.4

2

10.4 ± 6.7

14.5 ± 9.2

10

14.0 ± 14.7

11.9 ± 8.6

CD56+

8

7.4 ± 4.9

12.6 ± 8.5a

2

4.8 ± 2.8

6.8 ± 3.8

10

7.2 ± 5.0

11.9 ± 8.6a

  1. ap < 0.05 Paired T-tests of pre- and post-elutriation samples
  2. Values represent the mean ± SD