Fig. 3

Circulating tumor cells pool captures majority of trunk mutations and additional mutations from metastatic disease. a Variant allele frequencies for treatment-naïve, CRPC, and CTC1 mutations. b Average variant allele frequencies of trunk, branch, and leaf mutations in two tissue samples and one CTC sample. c Allele fraction in CRPC tissue of mutations identified in treatment-naïve tissue, and d vice versa. On the left are mutations identified in both samples. On the right are mutations identified in only sample. In d, many of the mutations not identified independently in treatment-naïve tissue had evidence they were present at low allele frequency. e Number of trunk mutations identified in three pooled CTC sequencing libraries. f Fraction of CTC mutations shared with other tissue samples