Fig. 5

Seven chemokines and growth factors were noted to be highly secreted in the conditioned media of JHU-011 and JHU-019. Of those, only recombinant IL-6 and PDGF isoforms AA, BB, and AB independently caused significant migration of MSCs to levels comparable to OPSCC cell line JHU-019 when compared to 1% BSA treated media (a). Neutralizing antibodies to all PDGF isoforms (b) and IL-6 (c) in the conditioned media of cell lines JHU-011, -012, and -019 and/or -022 resulted in a significant reduction in MSC migration. PDGFRα and PDGFRβ was expressed on MSCs and not JHU-011, -012, -019 or JHU-022. 3T3 cells were used as a negative control (d). Only blockade of PDGFRα but not PDGFRβ resulted in a significant reduction in JHU-011 induced MSC chemotaxis. Two MSC passes P3 and P5 were used to ensure MSC passage did not affect PDGFR expression. Each experiment was performed in triplicate for n ≥ 3 and unless where indicated (*p < 0.0001, **p < 0.03)