Fig. 2

Expression of CD39 and CD73 in NCx-MSCs and CeCa-MSCs. The expression of CD39 and CD73 ectonucleotidases was determined in NCx-MSC (n = 5) and CeCa-MSC (n = 5) cell membranes by flow cytometry analysis (a) and by immunocytochemical analysis (b, c) as described in “Methods” section. The arrows indicate typical cells stained with human anti-CD39 and anti-CD73 mAbs. The mean fluorescence intensity (MFI) ± SEM of 10,000 events (a), and the total expression density (TED) evaluated by digital pathology using the Aperio CS system (c) are shown. Secondary antibody alone was included as control (Ctl) for the experiments. The images were taken at ×20 magnification (Scale bar 100 µm). Asterisk indicates significant differences (P < 0.05) compared to NCx-MSCs