Fig. 5

Effects of EXE (20 min or 48 h) and exendin 9–39 on AS-160, TBCD1D and AMPK in differentiated L6 cells. L6 myotubes were stimulated for 20 min with 100 nmol/l EXE, 100 nmol/l exendin 9–39 or the combination of both. Where indicated cells were stimulated with 100 nmol/l INS for 30 min and 100 nmol/l EXE for 20 min or 48 h. The blots were probed with phospho-specific AS160 T642 (a), TBCD1 (b) and AMPKα T172 (c). To normalize for protein levels, the blots were stripped and re-probed with β-actin antibodies. Each bar represents the mean ± SD of three independent experiments. (*p < 0.001 and **p < 0.01 vs. control; ^#p < 0.02 vs. EXE)