Fig. 3

LNA-ISH of miR-142-5p in FFPE samples. A 5ʹ-FITC labeling LNA-ISH for miR-142-5p detection (×100). Green staining of miR-142-5p was visible in part of follicular epithelium. B–E 3ʹ,5ʹ-digoxin double labeling LNA-ISH for miR-142-5p detection. B Differential expression of miR-142-5p between normal thyroid and HT (×100). C The specific expression region of miR-142-5p in HT (bottom right ×200), including the ectopic lymphocyte (top left a ×1000), injured follicular epithelial cells (top right b ×1000) and normal follicular epithelium adjacent to the lesion (bottom left c ×1000). D Hematoxylin-eosin staining and LNA-ISH with serial section and the different intensity of miR-142-5p in HT (left and middle ×200, right ×1000). Case 1 showed mass-like positive signals and case 2 showed dot-like positive signals. E Expression patterns of miR-142-5p in various types of thyroid disease (×200). miR-142-5p is only positive in the HT with PTC cases