From: Clinical-scale expansion of mesenchymal stromal cells: a large banking experience
Criteria | Min. | Max. | Median |
---|---|---|---|
MSC culture parameters | |||
 Donor age (years) | 18 | 52 | 26.7 |
 BM sample volume (mL) | 25 | 70 | 50 |
 Mononuclear cells post-Ficoll (×106) | 124 | 956 | 280 |
 CFU-F (number per 2 × 106 cells) | 2 | 96 | 25 |
 Final MSC yields (×106) | 19 | 5431 | 546 |
 PDL (P1-harvest) | 2.38 | 7.18 | 4.69 |
 DT in hours (P1-harvest) | 46.8 | 141 | 71.7 |
 Viability (%) | 72 | 100 | 85 |
 Number of aliquots (=bags)/culture | 1 | 45 | 4 |
 Number of cells/aliquot (×106) | 19 | 189 | 110 |