Fig. 5

Change in EMT phenotype and morphology after PFN2-siRNA transfection in ESCC cells. a After silencing of PFN2 with PFN2-siRNA in ESCC cells, the protein levels of E-cadherin, Vimentin, Snail, Slug and ZEB1 were measured by Western blot. (Bar chart showing) Relative protein levels of E-cadherin, Vimentin, Snail, Slug and ZEB1 normalized to β-actin in b ECa109 cells, c EC9706 cells and d TE-1 cells. e Morphological change images of ECa109 cells after transfecting with PFN2-siRNA for 24, 36, and 48 h