Fig. 4
VEGF-B inhibited H/R-induced H9c2 cell apoptosis in vitro. a pAkt, pERK1 and pp38MAPK expression was detected using western blot, as described in the “Methods” section (b), and quantified via normalization to a-tubulin (b–d). *P < 0.05 vs. 15 % FBS-treated cells; # P < 0.05 vs. H/R-treated cells; @ P < 0.05 vs. 0.1 ng/ml VEGF-B-treated cells; $ P < 0.05 vs. 1.0 ng/ml VEGF-B-treated cells; & P < 0.05 vs. 10 ng/ml VEGF-B-treated cells; ^ P < 0.05 vs. 20 ng/ml VEGF-B-treated cells, n = 6. e Cell apoptosis was detected using Annexin V/PI staining, n = 6. f Quantitative analysis of cell apoptosis in (e). *P < 0.05 vs. 15 % FBS-treated cells; # P < 0.05 vs. H/R-treated cells; @ P > 0.05 vs. H/R-treated cells; $ P > 0.05 vs. H/R-treated cells; & P < 0.05 vs. H/R-treated cells; ^ P < 0.05 vs. 20 ng/ml VEGF-B-treated cells, n = 6