Fig. 6

Determination of autophagy proteins (LC3 & ATG7) in WT and Nrf2^−/− in response to HIES. a IF analysis of autophagy antibodies LC3 (1:500; v/v) and ATG7 (1:500; v/v) and double immunostaining of ATG7/Ubiquitin (1:500; v/v). Blue: nucleus (DAPI); Green: localizes LC3; Red/Green merge (Yellow): ATG7/Ubiquitin. b Fluorescence intensity was calculated as fold change of mean values of the WT sedentary group and the data is represented as mean ± SEM in the histograms (n = 3–4/group). *P < 0.05 vs SED WT; ^#P < 0.05 vs SED Nrf2^−/− and ^$P < 0.05 vs HIES WT