Fig. 6

Effects of PRPs on the osteogenic differentiation of HBMSCs. a mRNA expression of OC of HBMSCs was detected by RT-PCR after osteogenic differentiation induction for 14 days; b OC concentration in conditioned medium was detected by ELISA after osteogenic differentiation induction for 14 days; c mRNA expression of Runx2 of HBMSCs was detected by RT-PCR after osteogenic differentiation induction for 14 days; d Runx2 expression was detected by western blotting after osteogenic differentiation induction for 14 days; e alizarin red staining of HBMSCs after osteogenic differentiation induction for 21 days; f, alizarin red staining was quantified by a colorimetric assay and the absorbance value was measured at 450 nm. Compared with FBS, both L-PRP and P-PRP improved the osteogenic differentiation of HBMSCs, with P-PRP showing greater effects. Bars represent the means and standard deviation (n = 3), and scales represent 200 μm; * indicates the statistically significant difference between PRPs and FBS (P < 0.05); ^# indicates the statistically significant difference between P-PRP and L-PRP (P < 0.05)