Fig. 4

Infused ERCs increase the percentage of CD4⁺CD25⁺ Tregs in the spleen and contribute to the protection of kidney against IRI. a Flow cytometric analysis of splenic CD4⁺CD25⁺ Treg population was performed in the sham, IRI-untreated and ERC-treated groups. b ERC treatment significantly increased the percentage of Tregs in IRI mice compared with those of untreated IRI mice. Bar graphs represent mean ± SD of three separate experiments. p values were determined by one-way ANOVA. Data shown are a representative of three separate experiments performed (*p < 0.05, vs. IRI-untreated group, ^# p < 0.05, vs. sham group, n = 6)