Fig. 2

hD1R maintained quiescence and regeneration of human UCB CD34⁺ cells. CD34⁺ cells were cultured with or without HUVECs in the presence of h5GF plus hD1R. The cells were harvested on day 7. a Cultured cells were analyzed by using annexin V staining followed by FACS to determine the percentage of cell apoptosis (n = 4). b Cell-cycle analysis of CD34⁺ cells expanded in different conditions or freshly purified from UCB (Day 0). The number of cells in various stages of the cell cycle was quantified measuring the area under the red peaks (G0/G1). c Representative plots of cell-cycle analysis of CD34⁺ cells in (c). Bars = mean ± SD. *P < 0.05