Fig. 1
Human UCB CD34+ cells expansion by hD1R requires direct cellular interaction with live HUVECs. a CD34+ cells (2 × 103) were cultured with or without HUVECs in the presence of h5GF plus hD1R. The cells were harvested on day 7 and counted (n = 4). b, c The cells in (a) were analyzed by FACS to determine the percentage (b) and the number (c) of CD34+ cells (n = 4). d Representative FACS analysis of CD34+ cells in (b). e The cells (1 × 103) in (a) were used for colony-forming assay (n = 4). f Photomicrographs of human hemopoietic cell colonies. Scale bar represented 200 μm. Bars = mean ± SD. *P < 0.05