Fig. 2

Effects of FLP ointment on Cox-2 expression in the tumor specimens. a Immunohistochemical analysis of Cox-2 expression (×200 magnification and ×100 magnification). The brown or brown yellow represented positive Cox-2 protein. b A representative band of Cox-2 protein expression by Western blot analysis. c Relative protein level of Cox-2 (n = 3). Values were expressed as mean ± SD. **p < 0.01, *p < 0.05, ***p < 0.001, and ****p < 0.0001. FLP Fei-Liu-Ping ointment group, C celecoxib group, FLP + C Fei-Liu-Ping ointment plus celecoxib group. FLP ointment significantly inhibited the Cox-2 expression in tumor specimens at the initial stage without exerting time-dependent inhibitory effect. FLP ointment in combination with celecoxib did not show obvious advantage in inhibiting Cox-2 expression