Fig. 3

Effect of overexpression of Sirt1 on high glucose-stimulated HK-2 cells. Cells were pre-treated with pcDNA-Sirt1 with pcDNA as control. 48 h after incubation, cells were triggered with 5.5 mM and 45 mM glucose for 48 h. a Level of p65 acetylation, b translational activity of NF-κB and c cell viability were determined. Data were presented as mean ± SD. *P < 0.05 vs. cell treated with 5.5 mM glucose, ^#P < 0.05 vs. cell treated with 45 mM + pcDNA