Fig. 5

Effects of TNFα and IL-10 on GGT expression in monocytes. a Freshly isolated monocytes were incubated with TNFα (10 ng/ml) and/or IL-10 (20 ng/ml) in complete medium for 48 h. Results are mean ± SD of eight separate determinations. Data are expressed as mU/mg of cellular proteins and were analyzed by one-way ANOVA with Newman–Keuls test for multiple comparisons; ^§p < 0.001 as compared to untreated control; *p < 0.001 as compared with TNFα-treated samples. b Isolated monocytes were let to adhere for 24 h, then they were incubated with M-CSF (50 ng/ml) in the presence of an anti-IL10 antibody (1 μg/ml) or the corresponding isotype control for 6 days. Results are expressed as the ratio of GGT expressed by antibody-treated cells against M-CSF treatment alone (mean ± SD of seven separate determinations). Data were analyzed by Student’s t test for paired data; *p < 0.05